We observed that ASFV-Δ110-9L/505-7R infection increased HT-DNA-triggered transcription levels of Ifn-b (Fig. 4A), Isg56 (Fig. 4B), Cxcl10 (Fig. 4C), and Isg15 (Fig. 4D) compared with ASFV-WT. Here, ISG15 is linked to infection.