Furthermore, the results of RT-qPCR and WB indicated that there were downregulations of development-related genes (CCND1, PCNA), EMT-associated genes (N-cadherin, Vimentin) and anti-apoptotic BCL-2, while E-cadherin was upregulated in AGS/RNU12-OE or MGC803/RNU12-OE cells (Fig. 2H–K,R,S); These findings showed that RNU12 might inhibit the GC cell aggressiveness in vitro (the original blots in Fig. 2I,K,R,S are shown in Supplementary file 1). The gene discussed is CCND1; the disease is gastric cancer.