Furthermore, the results of RT-qPCR and WB indicated that there were downregulations of development-related genes (CCND1, PCNA), EMT-associated genes (N-cadherin, Vimentin) and anti-apoptotic BCL-2, while E-cadherin was upregulated in AGS/RNU12-OE or MGC803/RNU12-OE cells (Fig. 2H–K,R,S); These findings showed that RNU12 might inhibit the GC cell aggressiveness in vitro (the original blots in Fig. 2I,K,R,S are shown in Supplementary file 1). This evidence concerns the gene BCL2 and gastric cancer.