A systematic analysis of known, previously reported ALS variants was extended by using codon‐based analysis to look for neighbouring ALS‐associated variants and machine‐learning algorithms to look for splice site variation, yielding novel deleterious splice donor variants in TBK1 (c.1340 + 2 T > G) and in OPTN (c.1242 + 1G > A). Here, TBK1 is linked to amyotrophic lateral sclerosis.