To test whether VP7 interacts with HSP70 on the plasma membrane, we separated the fractions of cytoplasmic and plasma membrane from CIK lysates and incubated the fractions with nickel-nitrilotriacetic acid (Ni-NTA) beads coupled with recombinant VP7, His pulldown analysis confirmed that VP7 and HSP70 mainly colocalized on the plasma membrane of CIK cells, while GCRV infection increased their interaction on the membrane (Fig. 4G). The gene discussed is HSPA1A; the disease is infection.