To determine whether GCRV propagation and high expression of HSP70 were interrelated and temperature dependent, and endogenous HSP70 expression variation by temperature switch treatment mediates viral propagation, we first performed a temperature switch treatment, followed by GCRV infection (Fig. S2E), and the data showed that switches from 18°C to 28°C increased the HSP70 transcription and viral replication by a factor of 3 to 5, while switches from 28°C to 18°C dampened it by a factor of 7 to 9 (Fig. S2F and G). This evidence concerns the gene HSPA1A and infection.