HSPA1A and infection: To further verify that HSP70 upregulation by temperature stress could mediate GCRV entry, CIK cells cultured at 18°C were transiently switched to 28°C for 1 h, followed by GCRV infection, RT-PCR analysis showed that the temperature switch from 18°C to 28°C increased HSP70 transcription and GCRV genome entry by factors of 2 and 5, respectively.