Then, we detected the RHOT2 expression in human colon cancer cells (SW480, HT29, HCT-116, RKO, DLD1, and LoVo) by western blot and RHOT2 was confirmed to be expressed in all colon cancer cell lines (Figure 4F and Figure 4—source data 2). This evidence concerns the gene RHOT2 and malignant colon neoplasm.