Neuro2a (a mouse neuroblastoma cell line) cells treated with siRNAs targeting SNX32 (SNX32KD) and SNX6 (SNX6KD) (Figure 7—figure supplement 1A) were differentiated to form neurites by replacing the standard growth media (MEM with 10% FBS) with the differentiation media (MEM with 1% FBS containing 10 μmol/l retinoic acids) and incubated for 48 hr. The gene discussed is SNX6; the disease is neuroblastoma.