SLU7 and inflammatory bowel disease: Alternative splicing, which is regulated by RNA-binding proteins, is tissue specificity and identified by cis-acting splicing sequences.16 Alternative splicing studies are mainly used to monitor the mRNA expression at exonic resolution using RNA-seq under different experimental conditions, including knockdown, knockout, or overexpression of condition-specific splicing factors.17 Alternative splicing of pre-mRNA has been demonstrated to impact the pathogenesis and progression of inflammatory disease, exemplified by IBD.