To address this knowledge gap, subsequent studies of tumour‐infiltrating lymphocytes (TILs) using a C57 mouse model, including sequencing analysis, T‐cell receptor (TCR) detection, and flow cytometry, have revealed alterations in the TCR library, increased activation of antigen‐specific T lymphocytes as well as secretion of antitumour effector molecules (such as IFN‐γ and Granzyme B) following tumour rechallenge after cryoablation. The gene discussed is IFNG; the disease is neoplasm.