To further identify roles of MFAP5 in CAFs, we extracted and cultured human primary CAFs (CAF3) derived from the PDAC patient, and mouse primary CAF cell line (ImdyCAF) derived from spontaneous pancreatic tumors in transgenic mouse (KrasLSL-G12D, Trp53LSL-R172H, Pdx1-Cre (KPC) mice) (Fig. S3A). Here, PDX1 is linked to pancreatic neoplasm.