To further identify roles of MFAP5 in CAFs, we extracted and cultured human primary CAFs (CAF3) derived from the PDAC patient, and mouse primary CAF cell line (ImdyCAF) derived from spontaneous pancreatic tumors in transgenic mouse (KrasLSL-G12D, Trp53LSL-R172H, Pdx1-Cre (KPC) mice) (Fig. S3A). This evidence concerns the gene MFAP5 and pancreatic neoplasm.