Our scRNA-seq analysis identified a Lrat+Fbln2+ aHSC subpopulation as the putative source of CYP1B1-catalyzed 12-HHTrE generation, the finding that was supported by the presence of CYP1B1+ CAF in patient HCC via IHC and scRNA-seq analysis. This evidence concerns the gene LRAT and hepatocellular carcinoma.