However, our qPCR analysis revealed that the expression of 12-HHTrE biosynthetic genes (PTGS2, TBXAS1, CYP1B1), and YAP1 and LTB4R2 were all significantly upregulated in patient HCC vs. normal liver (Fig. 4c). The gene discussed is CYP1B1; the disease is hepatocellular carcinoma.