We also performed IHC for CYP1B1 and LTB4R2 in patient HCC sections and detected CYP1B1+ CAF surrounding LTB4R2+ HCC cells (Fig. 4d), a spatial relationship that supports aHSC-HCC crosstalk involving the CYP1B1-LTB4R2 pathway. This evidence concerns the gene CYP1B1 and hepatocellular carcinoma.