At Day 4 and Day 8 of Matrigel culture, ZO-1 (green) was present at the apical site, and E-cad (red) was present at the basolateral site of the cyst in both control (Fig 4A, c and f) and HA-MST3-KD cells (Fig 4A, n and q). This evidence concerns the gene STK24 and cyst.