When we compared DE genes of Aβ + apoE2 + FH and Aβ + apoE4 + FH samples to all samples we observed upregulation of genes (LMTK2, ATP1A1, FLOT1 and DNAJC5; Tiwari et al, 2015; Petrushanko et al, 2016; Abdullah et al, 2019; Bencze et al, 2019) that when downregulated could potentially promote AD pathogenesis (Fig 5B–D, Table 1, Appendix Table S5 and Dataset EV2). This evidence concerns the gene FLOT1 and Alzheimer disease.