As shown in Fig. 5a and b, NCKAP1 (green) accumulated at the F-actin-rich apical region of the cell membrane (represented as phalloidin, red) which formed a phagocytic cup during phagocytosis in ALS(S)-iMGs (white dot-lined box and white arrow). Here, NCKAP1 is linked to amyotrophic lateral sclerosis.