The in-depth research showed that AIF increased the release of lactate dehydrogenase (LDH), and upregulated the expression levels of NLRP3, caspase- 1, IL-18 and IL-1β, suggesting that AIF induced the pyroptosis of HCC cells through activating NLRP3-caspase-1-IL-1β pathways. The gene discussed is IL1B; the disease is hepatocellular carcinoma.