However, the luciferase activity of the plasmid containing the mutant fragment of NLRP3 3′-UTR was not influenced by co-transfection with the miR-223 analog, indicating that miR-223-3p directly interacted with 3′- UTR of NLRP3 mRNA.Further experiments showed that after transfection with miR-223-3p, the expression of NLRP3, caspase-1, IL-1β and IL-18 in HCC cells was inhibited, indicating that miR-223-3p suppressed NLRP3 inflammasome. The gene discussed is IL1B; the disease is hepatocellular carcinoma.