Conversely, the articles dealing with the neuroprotective effects of HO-2 were performed in adult HO-2 null mice, exposed to middle-cerebral artery occlusion (1 h occlusion followed by 23 h reperfusion) or collagenase injection, to mimic ischemia or hemorrhage, respectively, and whose brains were removed and analyzed just after the end of the experiments without any further in vitro manipulation. Here, HMOX2 is linked to ischemia.