To further investigate whether the redistribution of fascin and β-catenin is related to the malignancy of cervical tissue lesions, three different types of cervical cancer patient tissue samples, including normal (HR-HPV negative for intraepithelial lesion or malignancy, NILM), high-grade squamous intraepithelial lesion (HSIL) and cervical squamous cell carcinoma (CC), were subjected to immunohistochemical analysis by staining with fascin and β-catenin with tissue panoramic scanning and quantification. This evidence concerns the gene FSCN1 and cervical cancer.