To determine whether NEDD4L-TXNIP-CHOP axis drives NASH development in hepatocytes in vivo, we first measured the expression levels of TXNIP, CHOP, and NEDD4L in hepatocytes and NPCs from the livers of mice fed either a normal diet or an MCD diet. This evidence concerns the gene NEDD4L and metabolic dysfunction-associated steatohepatitis.