Notably, flow cytometry analysis of Ki-67, a well-known marker of cell proliferation (Sun and Kaufman, 2018), showed a significant reduction in the Ki-67 positive sub-population in ClK cells compared with the high-grade astrocytoma T98G cell line as well as a decreasing trend according to cell confluence. The gene discussed is MKI67; the disease is astrocytoma (excluding glioblastoma).