Given the high proportion of CD4+ TILs exhibiting an effector memory phenotype (CD44HICD62L-) in intermediate and late-stage tumours (D14 & D21; Figure 6A), these data suggest that as MC38 tumours progress, they are infiltrated by CD4+ effector subsets other than TH1 and TREG cells, likely TH2 or TH17 cells. Here, CD4 is linked to neoplasm.