The composition of PD-1 and TIM-3 subsets within CD4+ TILs remained relatively stable throughout the course of MC38 tumour progression, with distinct PD-1-TIM-3- (34-44%), PD-1+TIM-3- (24-26%) and PD-1+TIM-3+ (29-39%) populations apparent (Figures 8A, B). Here, CD4 is linked to neoplasm.