These results also indicating that BL/LL patients derived Tregs have more suppressive activity than Tregs of BT/TT leprosy patients and it may be IL-10 dependent.With this intend we estimated the IL-10 cytokine in the culture supernatant of co-culture of Tresponder and Trregs with and without blocking of PD-1. The gene discussed is IL10; the disease is leprosy.