To explore the USP10 expressions at different times and the effect of VNS on its expression after ischaemic stroke, the protein and mRNA levels in the ischaemic penumbra of the cerebral cortex were assessed by western blot and RT-qPCR, respectively within 7 days following reperfusion (Figures 1A, B, D). This evidence concerns the gene USP10 and ischemic stroke.