Flow cytometry-based analysis showed that the surface expression of TLR4 was reduced upon infection and TAK-242 (1μM) treatment, significantly [from 75.87 ± 1.247 to 51.07 ± 0.6360% (CHIKV) and 53.5 ± 0.611% (TAK-242)] (Figure S5C). This evidence concerns the gene TLR4 and infection.