Starting from its discovery, TLR4 has been known to play a critical role to study the functional aspects of host-pathogen interactions and associated pro-inflammatory immune responses, thus it has evolved as a suitable target for modern-age bio-medical research in the field of rheumatoid arthritis, necrotizing enterocolitis, and inflammatory bowel disease (25, 27, 28). This evidence concerns the gene TLR4 and necrotizing enterocolitis.