Additionally, both isolated adipocytes and myocytes exposed to H2O2 demonstrate impaired PKB activation (Tirosh et al., 1999; JeBailey et al., 2007) and markers of insulin-signaling (Rudich et al., 1997; Tirosh et al., 2001; JeBailey et al., 2007), while conversely the use of antioxidants (e.g. lipoic acid or N-acetylcysteine) prevent the induction of insulin resistance (Rudich et al., 1999; JeBailey et al., 2007). This evidence concerns the gene INS and Insulin resistance.