PRRT2 and Insulin resistance: The importance of DAG-mediated PKC activation in this pathway has been evidenced with the ablation of PKC-θ in murine skeletal muscle which protects mice from high-fat diet-induced insulin resistance (Kim et al., 2004), as does muscle-specific alanine substitutions on IRS-1 at various serine phosphorylation sites (Morino et al., 2008).