In our experiment, a single FUS-Cav treatment demonstrated a minor inhibitory effect on the expression of SRD5A1 and SRD5A3 in prostate cancer cells, and the combination treatment of FUS-Cav and water bath HT led to a significant reduction of SRD5A protein compared to single HT, but the suppressive effects of combination treatment for an isoform of SRD5A are cell-type dependent. Here, SRD5A1 is linked to Familial prostate cancer.