The most upregulated genes in the tumor group included EN1, PRAME, FNDC1, APBA2, TBX1, FABP7, CBP2B, and TTYH1, while CST2, SMR3B, KLK1, CTBS, THN1, and STATH were significantly decreased, as shown in the volcano plot (Figure 1D). The gene discussed is EN1; the disease is neoplasm.