It was also observed that the methylation level of NALCN promoter was increased in each stage tumor tissues of BRCA, CESC, COAD, HNSC, KIRC, and READ (Figure 9B), and any lymph node metastasis status of BRCA, CESC, COAD, HNSC, LUAD, LUSC, PAAD, PRAD, and READ, than those in normal tissues (Figure 9C). The gene discussed is NALCN; the disease is neoplasm.