A study of breast cancer cells mentioned that rigid ECM can directly stimulate integrin β1 and FAK, accelerate FAs maturation, and induce activation of RhoA/ROCK1/p-MLC and RhoA/ROCK2/p-cofilin signaling cascades, while ROCK1 phosphorylated myosin can regulate light chain and promote traction force generation, and ROCK2 phosphorylated cofilin inhibits F-actin depolymerization to remodel cytoskeleton and finally regulate cell migration (Peng et al., 2019). The gene discussed is RHOA; the disease is breast cancer.