The combined results of LVEF and cardiac biomarkers (NT-proBNP, TNF-α and IL-6) indicated that CHF rats treated with 5 min-, 5 cycles-, 8 weeks-, and 12 weeks-PIT had a significant improved cardioprotection compared to no-treatment group, while no significant results on LVEF between 8 weeks- and 12 weeks-PIT was observed. This evidence concerns the gene TNF and congestive heart failure.