Although the establishment of an organoid requires an advanced organ culture model to form a pancreatic niche in vitro from the porcine bladder (PUB) (Melzer et al., 2022), it cannot acquire better experiment repeatability and confirm that every cell contained a genetic mutation, such as KRAS. Thus, to accurately simulate the pancreatic cancer microenvironment, a pancreatic model that contains stable KRAS mutations and can reveal the development of additional KRAS point mutations after the introduction of a certain treatment is required. The gene discussed is KRAS; the disease is pancreatic neoplasm.