After inclusion of these variables in the multivariate analysis, it was found that the neutrophil-lymphocyte ratio (NLR)>2.4, HBV-DNA>2000 IU/ml, AFP>400 ng/ml, narrow surgical margins, tumor diameter, multiple tumors, and the absence of postoperative TACE independently predicted both recurrence and OS (Tables 2, 3). The gene discussed is AFP; the disease is neoplasm.