Next, we detected four genes with differential expression greater than 10-fold by Western blot at the cellular and tissue levels, namely, MAP1LC3A, OLFM1, CEND1, and CLTB. The results showed that the expression levels of the above proteins were significantly upregulated relative to both glioma paracancerous glial cells and glioma paracancerous glial tissue. The gene discussed is CEND1; the disease is central nervous system cancer.