Using DSP technology, we screened 20 prognostic gene markers located in tumor cell-enriched regions (CEP85L, KRT5, MAST4, MYO1G, SLA, SMARCC2, UPK3B, ATF5, BEX3, BIK, CADM4, CDK2AP1, CLDN1, DLL4, IGFBP2, NFE2L3, PTGS2, SMC1A, SUSD4 and VRK2) and 4 prognostic markers in immune cell-enriched regions (CALHM2, CCL21, FCGR2C and SLC8A1). The gene discussed is CLDN1; the disease is neoplasm.