As shown in Figure 6B, liver cancer cells on high matrix stiffness at 16 kPa had increased free fatty acid levels in the media (p < 0.01, Figure 6B, Figure S2D) and markedly downregulated mRNA and protein levels of ACADL compared with the control group (p < 0.01; Figure 6C, D and Figure S2E, F). Here, ACADL is linked to liver cancer.