To further validate these observations, the nuclear and cytoplasmic proteins from three cases of fresh frozen CRC and pericarcinoma tissues were isolated for Western blot analysis, exhibiting higher BATF2 expression in the cytoplasm of tumour tissues than that in pericarcinoma (Figure 1D), which was also observed in human CRC cells (HCT116) compared with normal colon epithelial cells (CCD 841 CoN) (Figure 1E–G). This evidence concerns the gene BATF2 and neoplasm.