To investigate the availability of intracellular cavitation-mediated nuclear gene delivery for other functional genes, we created pCMV-IRES-mCherry (pCherry) or pCMV-E-cadherin-IRES-mCherry (pEcad) plasmids and tested their effects on invasion and migration of C6 tumor cells via intracellular cavitation in a time-controlled manner (Supplementary Fig. 5a). This evidence concerns the gene CDH1 and neoplasm.