We performed RNA sequencing (RNA-seq) of pCherry-GVs@C6 cells and pEcad-GVs@C6 cells treated with acoustic irradiation at the SynG2/M phase to investigate the mechanisms by which overexpression of E-cadherin via acoustically triggered nuclear gene delivery enhanced the inhibition effects against tumor invasion and migration. This evidence concerns the gene CDH1 and neoplasm.