We synthesized the B7-H3 scFv sequence based on 8H9 clone and constructed the second-generation B7-H3 CAR containing CD8α transmembrane region, CD28 intracellular costimulatory domain, and CD3ζ intracellular signaling domain after confirming that B7-H3 is highly expressed in prostate cancer (Fig. 2A). The gene discussed is CD28; the disease is Familial prostate cancer.