Taken together, the present study revealed the crucial function of WWP2 and the fundamental importance of the JNK-WWP2-DDX3X regulatory axis in T2DM-induced vascular endothelial injury, suggesting that JNK-WWP2-DDX3X axis has potential as a preventive and therapeutic target for DVCs. The gene discussed is DDX3X; the disease is type 2 diabetes mellitus.