Identically, reduced protein levels were shown in MSI2-overexpressing AML cells when they were transfected with miR-143, indicating that miR-143 to some extent prevented MSI2 from activating the Notch1 signaling pathway and cancer stemness-related genes (Fig. 5G and Additional file 1: Fig. S9C–E), and it also partially reversed the expression of migration-related proteins regulated by MSI2 (Fig. 5H and Additional file 1: Fig. S9F). This evidence concerns the gene NOTCH1 and acute myeloid leukemia.