Furthermore, differentially expressed genes (DEGs) from GEO Datasets (GSE22775) were identified, of which 250 genes were upregulated and 371 genes were downregulated when AML cell lines were treated with MSI2 shRNA (Fig. 2F). And GO pathway analyses identified enriched pathways in DEGs, including cell activation, migration, apoptosis and proliferation (Fig. 2G). This evidence concerns the gene MSI2 and acute myeloid leukemia.