NOTCH1 and neoplasm: To determine whether miR-143 works as a tumor suppressor to reverse the carcinogenic effects of MSI2, western blotting assays were performed to verify that the expression of key factors of Notch1 signaling pathway were affected negatively by miR-143 mimic in HEL and HL-60 cells, as well as migration-relative proteins in MiR-143 vector-transfecting HEL cells (Fig. 5A–C).