A study using genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screening discovered that Ikaros family zinc finger protein 2 (IKZF2) and transducin-like enhancer of split 4 (TLE4) may be linked with the functional suppression and exhaustion of T cells, thus affecting the killing effects of CAR T-cells on GBM stem cells. This evidence concerns the gene TLE4 and glioblastoma.