Unfortunately, previous studies that investigated the ability of Wnt/PCP components to specifically engage and regulate Rho GTPases in cancer cells have predominantly assessed global GTPase activity in lysed cells via GST pull-down assays [23, 24, 32], leaving both the localization and kinetics of Wnt/PCP-mediated RhoA and Rac1 activity and the contribution of Wnt/PCP components to leader cell biology largely unexplored. The gene discussed is RAC1; the disease is cancer.