Effective deletion of Vangl2 in mammary tumors of Vangl2fl/fl;MMTV-Cre±;MMTV-NDL± (Vangl2fl/fl/NDL) mice relative to Vangl2fl/fl;MMTV-NDL± (Vangl2+/+/NDL) mice was confirmed by qPCR (Additional file 4: Fig. S8b). The gene discussed is VANGL2; the disease is breast cancer.