To clarify the association between DVL3 and CSLCs properties of CRC, we performed a sphere formation assay and found that the expressions of DVL3, stem cell surface markers (CD44 and CD133) and stemness-associated transcription factor (SOX2 and c-Myc) were stronger in CSLCs-enriched tumorspheres than that in monolayer-cultured HCT-8 and SW620 cells (Fig. 4A, B). This evidence concerns the gene CD44 and colorectal carcinoma.