In the current study, we bred the ZKSCAN3f/f mice with mice carrying cardiomyocyte‐specific cre and generated cardiomyocyte specific ZKSCAN3 knockout (Z3K) mouse to determine function of ZKSCAN3 in the heart, and its role in cardiac remodeling in response to pressure‐overload induced by transverse aortic constriction (TAC). The gene discussed is ZKSCAN3; the disease is persistent truncus arteriosus.