PDCD1 and non-small cell lung carcinoma: Mass cytometry has been used for the assessment of the TME in NSCLC where CyTOF was used in conjunction with multiplexed IF to define the significance of PD-1, LAG-3 and T cell immunoglobulin and mucin domain-containing protein 3 (TIM-3) expression in tumour infiltrating lymphocytes (72).