Wu et al. (2014) developed an ECL imaging strategy for the simultaneous detection of multiple cancer biomarkers, including AFP, based on a closed bipolar electrode array (Wu et al., 2015). In addition, Zhang et al. (2015) described an ECL method based on DNA aptamer–target recognition and T7 exonuclease-assisted cycling signal amplification for VEGF-165 detection. Consequently, the ECL-based aptasensor had a detection limit of 0.2 pM within a wide range of VEGF-165 concentrations of 1 pM–20 nM (Zhang et al., 2015). Here, AFP is linked to cancer.