The livers of C57BL/6 mice inoculated with 10,000 SPZ of either IL-6 Tg-PbANKA/LISP2 or WT PbANKA parasites were harvested at 48h post-infection and were subjected to RT-qPCR using primers designed to detect the codon-optimized parasite-encoded IL-6 transgene. This evidence concerns the gene IL6 and infection.