Indeed, dosage of IL-6 cytokine in supernatants collected 48h after infection of HepG2 cell cultures with IL-6 Tg-PbANKA/LISP2, IL-6 Tg-PbANKA/UIS4 or WT PbANKA SPZ, showed that IL-6 was detectable only in supernatants from IL-6 Tg-PbANKA/LISP2-infected HepG2 cultures, but not in IL-6 Tg-PbANKA/UIS4 or from PbANKA-infected HepG2 cell cultures (Supplementary Figure S2E). Here, IL6 is linked to infection.