Based on boxplot analysis, the mRNA levels of EIF2S1, CAV1, RAN, SELE, ANXA5, CYCS, CFL1, HSP90AA1, PKM, HSPA5, HNRNPH1, and DDX17 exhibited a significant overexpression in HNSCC than in healthy controls. This evidence concerns the gene CFL1 and head and neck squamous cell carcinoma.