Double immunofluorescence staining revealed an increase in the colocalization of LAMP1 with Aβ42 and p-Tau after TFEB overexpression in T2DM mice or HG-cultured HT22 cells compared to those transduced with the empty vector (Additional file 2: Fig. S2A, B, Fig. 6B, E, F, I), indicating successful induction of ALP-targeted clearance of AD-related proteins by TFEB overexpression. This evidence concerns the gene MAPT and Alzheimer disease.