Combined treatment of 3-MA and TFEB vector did not improve cognition in T2DM mice (Fig. 4F–I) or protect against cell apoptosis in HG-cultured HT22 cells (Fig. 7A), further supporting the idea that the therapeutic effect of TFEB overexpression on DE is mediated through ALP activation. The gene discussed is TFEB; the disease is type 2 diabetes mellitus.