Of these, 6 proteins are earlier reported to be associated with LN metastasis in GBC (KRT7, KRT19, VIM, PKM, LASP-1, TYMP), 33 proteins are associated with LN metastasis in other cancers (NPM1, SRI, FABP5, PPIA, HSPA9, AOC3, ANXA2, ANXA5, ANXA10, TAGLN2, HNRNPK, CAPG) while 19 proteins (DLST, MDH2, CKMT1A, APOC3, RDH16, VCAN) are novel to LN metastasis in cancer (Supplementary Table S4). This evidence concerns the gene ANXA2 and cancer.