To assess how the mTOR signaling pathway is highly activated by Kmt2d deficiency and given that KMT2D, as a histone lysine N‐methyltransferase, is required for H3K4 mono‐ and di‐methylation, we performed the cleavage under targets and tagmentation (CUT&Tag) technique to investigate the corresponding H3K4me1, H3K4me2, or H3K27ac levels in Kmt2d restored and knockdown AML cells. Here, MTOR is linked to acute myeloid leukemia.