FBL and acute myeloid leukemia: Studies have shown the connection between structural and functional alterations of nucleoli and tumorigenesis, where increased ribosome biogenesis is associated with larger nucleoli and malignant tumors generally have larger ones.[35, 36] From the analysis of transmission electron microscopic (TEM) images and immunofluorescence staining using an antibody against nucleolar protein fibrillarin, shKmt2d AML cells contained significantly enlarged nucleoli and brighter ribosome biogenesis key molecule fibrillarin staining, compared to control cells (Figure 3D,E).