By comparing the KMT2D binding and histone modification levels between Kmt2d restored and knockdown AML cells, we found that 2516 peaks had a significant decrease of KMT2D binding levels and contained reduced H3K4me1, H3K4me2, and H3K27ac modifications in Kmt2d‐deficient AML cells, which indicated these regions were specifically bound by KMT2D (p < 0.05, log2‐fold change < −1; Figure S4G and Table S4, Supporting Information). This evidence concerns the gene KMT2D and acute myeloid leukemia.