In order to confirm if miR-186-3p contributed to the NSCLC progression by altering KIF2C, A549-DDP cells were co-transfected with pcDNA-KIF2C and miR-186-3p mimics, and A549 cells were co-transfected with si-KIF2C and miR-186-3p inhibitors. The gene discussed is KIF2C; the disease is non-small cell lung carcinoma.